THE BIOSYNTHESIS OF HISTIDINE; l-HISTIDINOL PHOSPHATE PHOSPHATASE
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چکیده
منابع مشابه
Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis.
The deduced product of the Bacillus subtilis ytvP gene is similar to that of ORF13, a gene of unknown function in the Lactococcus lactis histidine biosynthesis operon. A B. subtilis ytvP mutant was auxotrophic for histidine. The only enzyme of the histidine biosynthesis pathway that remained uncharacterized in B. subtilis was histidinol phosphate phosphatase (HolPase), catalyzing the penultimat...
متن کاملStructural and mechanistic characterization of L-histidinol phosphate phosphatase from the polymerase and histidinol phosphatase family of proteins.
L-Histidinol phosphate phosphatase (HPP) catalyzes the hydrolysis of L-histidinol phosphate to L-histidinol and inorganic phosphate, the penultimate step in the biosynthesis of L-histidine. HPP from the polymerase and histidinol phosphatase (PHP) family of proteins possesses a trinuclear active site and a distorted (β/α)(7)-barrel protein fold. This group of enzymes is closely related to the am...
متن کاملThe separation and partial characterization of L-histidinol phosphatase and an alkaline phosphatase of Saccharomyces cerevisiae.
Two enzymes which effected the hydrolysis of the histidine biosynthetic intermediate histidinol phosphate were isolated from Saccharomyces cerevisiae. The enzymes were separated by diethylaminoethyl Sephadex column chromatography and partially characterized. The histidinol phosphatase was shown to be a speci6c enzyme, hydrolyzing only the histidine intermediate, histidinol phosphate. It was ins...
متن کاملNovel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases.
The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD...
متن کاملThe biosynthesis of histidine; D-erythro-imidazoleglycerol phosphate dehydrase.
Reaction 2 is catalyzed by the enzyme imidazoleacetol phosphate transaminase (1). Reaction 3 is catalyzed by the enzyme L-histidinol phosphate phosphatase (2). Reaction 4 is catalyzed by the enzyme L-histidinol dehydrogenase (3, 4). This report is concerned with the purification and description of the enzyme from N. crassa catalyzing Reaction 1, the dehydration of D-erythroimidazoleglycerol pho...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70840-5